Cullin 2 Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-4209R-Biotin |
| Product Name | Cullin 2 Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cullin 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 8453 |
| Subcellular location | Nucleus |
| Synonyms | Cullin2; Cullin-2; CUL 2; MGC131970; CUL2_HUMAN. |
| Background | Cullin 2 is a member of the family of human Cullin genes (CUL1, 2, 3, 4a, 4b and 5) homologous to the S. cerevisiae cdc53 gene. It is a component of E3 ubiquitin ligase complexes, including the inactive transcriptional elongation complex SIII, which mediates the ubiquitination of hypoxia-inducible factor (HIF). SIII is formed by three subunits: Elongin C, Elongin B and VHL. Cullin 2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Cullin 2 Polyclonal Antibody, Biotin Conjugated (bs-4209R-Biotin) validated for, and at what dilution?
bs-4209R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-4209R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cullin 2 Polyclonal Antibody, Biotin Conjugated (bs-4209R-Biotin)?
KLH conjugated synthetic peptide derived from human Cullin 2