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C9orf156 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Pig
  • Horse
Overview
Catalog # bs-4210R-Cy3
Product Name C9orf156 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Pig, Horse
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human C9orf156
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 51531
Swiss Prot Q9BU70
Subcellular location Nucleus
Synonyms NAP1; HSPC219; Nef-associated protein 1; Thioesterase NAP1; C9orf156
Background Hydrolyzes acyl-CoA thioesters (in vitro). Has a preference for substrates with medium chain length (C1-C14). Inactive towards substrates with C18 or C2 aliphatic chains. Its physiological substrate is not known.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is C9orf156 Polyclonal Antibody, Cy3 Conjugated (bs-4210R-Cy3) validated for, and at what dilution?

bs-4210R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does C9orf156 Polyclonal Antibody, Cy3 Conjugated (bs-4210R-Cy3) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Pig, Horse.

How should bs-4210R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise C9orf156 Polyclonal Antibody, Cy3 Conjugated (bs-4210R-Cy3)?

KLH conjugated synthetic peptide derived from human C9orf156

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4210R-Cy3)?

Cy3 on bs-4210R-Cy3: excitation / emission 512,550nm/570,615nm.