LARG Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-4213R-BF680-100uL |
| Product Name | LARG Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LARG |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23365 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ARHGEF12; KIAA0382; leukemia-associated rho guanine nucleotide exchange factor; Leukemia-associated RhoGEF; PRO2792; Rho guanine exchange factor GEF 12; Rho guanine nucleotide exchange factor GEF 12; Rho guanine nucleotide exchange factor 12; Similar to Mouse Lsc oncogene; ARHGC_HUMAN. |
| Background | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein is observed to form myeloid/lymphoid fusion partner in acute myeloid leukemia (from EntrezGene). LARG has also been implicated in linking heterotrimeric G proteins to microtubule organisation in regulation of cell polarity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is LARG Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4213R-BF680) validated for, and at what dilution?
bs-4213R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-4213R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LARG Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4213R-BF680)?
KLH conjugated synthetic peptide derived from human LARG
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-4213R-BF680)?
AbBy Fluor-680 on bs-4213R-BF680: excitation / emission 679nm/702nm.