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ACOT13 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACOT13) Polyclonal Antibody, Unconjugated (bs-42157R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACOT13) Polyclonal Antibody, Unconjugated (bs-42157R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACOT13) Polyclonal Antibody, Unconjugated (bs-42157R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACOT13) Polyclonal Antibody, Unconjugated (bs-42157R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACOT13) Polyclonal Antibody, Unconjugated (bs-42157R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACOT13) Polyclonal Antibody, Unconjugated (bs-42157R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Horse
  • Rabbit
Overview
Catalog # bs-42157r
Product Name ACOT13 Polyclonal Antibody
Applications
IHC-P IHC-F IF
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human ACOT13 protein
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 55856
Swiss Prot Q9NPJ3
Subcellular location Cytoplasm, Nucleus
Synonyms ACO13_HUMAN; Acyl-coenzyme A thioesterase 13; THEM2; EC:3.1.2.-; Acyl-CoA thioesterase 13; Hotdog-fold thioesterase superfamily member 2; Palmitoyl-CoA hydrolase; Thioesterase superfamily member 2; Acyl-coenzyme A thioesterase 13, N-terminally processed; HT012; PNAS-27; PNAS27; PNAS 27;
Application Dilution
IHC-P =1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500

Frequently asked questions

Which applications is ACOT13 Polyclonal Antibody (bs-42157R) validated for, and at what dilution?

bs-42157R is validated for IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ACOT13 Polyclonal Antibody (bs-42157R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Horse, Rabbit.

How should bs-42157R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ACOT13 Polyclonal Antibody (bs-42157R)?

Recombinant human ACOT13 protein

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