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TBL1X/SMAP55 Polyclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-4219R-BF405-100uL
Product Name TBL1X/SMAP55 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity 100% sequence similarity with TBL1Y
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TBL1X
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6907, 90665
Subcellular location Nucleus
Synonyms TBL1; EBI; F box like/WD repeat protein TBL1X; SMAP 55; SMAP55; TBL 1; TBL1X; Transducin beta like 1; Transducin beta like 1 X linked; Transducin beta like 1 X linked; Transducin beta like 1X; Transducin beta like 1X protein; TBL1X_HUMAN; F-box-like/WD repeat-containing protein TBL1X; Full=Transducin beta-like protein 1X; Transducin-beta-like protein 1, X-linked..
Background The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation. This encoded protein is found as a subunit in corepressor SMRT (silencing mediator for retinoid and thyroid receptors) complex along with histone deacetylase 3 protein. This gene is located adjacent to the ocular albinism gene and it is thought to be involved in the pathogenesis of the ocular albinism with late-onset sensorineural deafness phenotype. Four transcript variants encoding two different isoforms have been found for this gene. This gene is highly similar to the Y chromosome TBL1Y gene. [provided by RefSeq].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TBL1X/SMAP55 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-4219R-BF405) validated for, and at what dilution?

bs-4219R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TBL1X/SMAP55 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-4219R-BF405) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Horse, Chicken, Rabbit.

How should bs-4219R-BF405 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TBL1X/SMAP55 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-4219R-BF405)?

KLH conjugated synthetic peptide derived from human TBL1X

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-4219R-BF405)?

AbBy Fluor-405 on bs-4219R-BF405: excitation / emission 401nm/421nm.

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