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TM7SF2 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Sheep
  • Rabbit
Overview
Catalog # bs-4221R-Cy3
Product Name TM7SF2 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Sheep, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TM7SF2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7108
Subcellular location Cytoplasm
Synonyms ANG1; Another new gene 1 protein; DHCR14A; C 14 sterol reductase; C-14 sterol reductase; Delta 14 SR; Delta14 sterol reductase; Delta14-sterol reductase; Delta-14-SR; DHCR14A; ERG24_HUMAN; NET47; Putative sterol reductase SR 1; Putative sterol reductase SR-1; Sterol C14 reductase; Sterol C14-reductase; TM7SF2; Transmembrane 7 superfamily member 2.
Background Involved in the conversion of lanosterol to cholesterol.Expressed in adult heart, brain, pancreas, lung, liver, skeletal muscle, kidney, ovary, prostate, and testis, but not detected in placenta, spleen, thymus, small intestine, colon (mucosal lining), or peripheral blood leukocytes.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TM7SF2 Polyclonal Antibody, Cy3 Conjugated (bs-4221R-Cy3) validated for, and at what dilution?

bs-4221R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TM7SF2 Polyclonal Antibody, Cy3 Conjugated (bs-4221R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Rabbit.

How should bs-4221R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TM7SF2 Polyclonal Antibody, Cy3 Conjugated (bs-4221R-Cy3)?

KLH conjugated synthetic peptide derived from human TM7SF2

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4221R-Cy3)?

Cy3 on bs-4221R-Cy3: excitation / emission 512,550nm/570,615nm.