HSPG2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-42231R-Cy3-100uL |
| Product Name | HSPG2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Recombinant human HSPG2 protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3339 |
| Swiss Prot | P98160 |
| Subcellular location | Extracellular, Secreted, Cell membrane |
| Synonyms | Heparan Sulphate Proteoglycan; Perlecan; Basement membrane specific heparan sulfate proteoglycan core protein; Endorepellin (domain V region); Heparan Sulfate Proteoglycan; Heparan sulfate proteoglycan of basement membrane; HSPG 2; HSPG; Hspg2; LG3 peptide; Perlecan; PLC antibody Schwartz Jampel syndrome 1 (chondrodystrophic myotonia); SJA antibody SJS antibody SJS1 antibody. |
| Background | This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans(heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and Transthyretin, etc. and plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and Tardive dyskinesia.[provided by RefSeq, Mar 2010]. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is HSPG2 Polyclonal Antibody, Cy3 Conjugated (bs-42231R-Cy3) validated for, and at what dilution?
bs-42231R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSPG2 Polyclonal Antibody, Cy3 Conjugated (bs-42231R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-42231R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSPG2 Polyclonal Antibody, Cy3 Conjugated (bs-42231R-Cy3)?
Recombinant human HSPG2 protein
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-42231R-Cy3)?
Cy3 on bs-42231R-Cy3: excitation / emission 512,550nm/570,615nm.