UGT1A9 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4224R-Cy5.5-100uL |
| Product Name | UGT1A9 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UGT1A9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54600 |
| Subcellular location | Cytoplasm |
| Synonyms | GNT1; HLUGP4; LUGP4; UDP glucuronosyltransferase 1A9; UDPGT; UGT1I; UD19_HUMAN. |
| Background | UGT1A9 is a UDP glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N termini and identical C termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. UGT1A9 is active on phenols. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is UGT1A9 Polyclonal Antibody, Cy5.5 Conjugated (BS-4224R-CY5-5) validated for, and at what dilution?
BS-4224R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UGT1A9 Polyclonal Antibody, Cy5.5 Conjugated (BS-4224R-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should BS-4224R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UGT1A9 Polyclonal Antibody, Cy5.5 Conjugated (BS-4224R-CY5-5)?
KLH conjugated synthetic peptide derived from human UGT1A9
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4224R-CY5-5)?
Cy5.5 on BS-4224R-CY5-5: excitation / emission 675nm/694nm.