VAPA Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4226R-Cy5.5-100uL |
| Product Name | VAPA Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VAPA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9218 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | VAMP A; VAMP associated protein A; VAP 33; VAP A; VAP33; Vesicle associated membrane protein associated protein A; VAPA_HUMAN. |
| Background | VAPA is a type IV membrane protein, present in the plasma membrane, intracellular vesicles and may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Two transcript variants encoding distinct isoforms have been identified. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is VAPA Polyclonal Antibody, Cy5.5 Conjugated (BS-4226R-CY5-5) validated for, and at what dilution?
BS-4226R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VAPA Polyclonal Antibody, Cy5.5 Conjugated (BS-4226R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Rabbit.
How should BS-4226R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VAPA Polyclonal Antibody, Cy5.5 Conjugated (BS-4226R-CY5-5)?
KLH conjugated synthetic peptide derived from human VAPA
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4226R-CY5-5)?
Cy5.5 on BS-4226R-CY5-5: excitation / emission 675nm/694nm.