ZDHHC21 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-4229R-APC-Cy7-100uL |
| Product Name | ZDHHC21 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZDHHC21 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 340481 |
| Swiss Prot | Q8IVQ6 |
| Subcellular location | Cell membrane |
| Synonyms | DNZ1; DHHC21; DHHC-21; HSPC097; 9130404H11Rik; Palmitoyltransferase ZDHHC21; Zinc finger DHHC domain-containing protein 21; ZDHHC21 |
| Background | Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane. This affects rapid intracellular signaling by sex hormones via ERK and AKT kinases and the generation of cAMP, but does not affect that mediated by their nuclear receptor (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZDHHC21 Polyclonal Antibody, APC-Cy7 Conjugated (bs-4229R-APC-Cy7) validated for, and at what dilution?
bs-4229R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-4229R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZDHHC21 Polyclonal Antibody, APC-Cy7 Conjugated (bs-4229R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human ZDHHC21
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-4229R-APC-Cy7)?
APC-Cy7 on bs-4229R-APC-Cy7: excitation / emission 650nm/780nm.