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ADORA1 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-4235R-Cy5.5-100uL
Product Name ADORA1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Pig, Horse, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ADORA1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 134
Swiss Prot P30542
Subcellular location Cell membrane
Synonyms RDC7; Adenosine receptor A1; ADORA1
Background Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ADORA1 Polyclonal Antibody, Cy5.5 Conjugated (BS-4235R-CY5-5) validated for, and at what dilution?

BS-4235R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADORA1 Polyclonal Antibody, Cy5.5 Conjugated (BS-4235R-CY5-5) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Pig, Horse, Rabbit.

How should BS-4235R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADORA1 Polyclonal Antibody, Cy5.5 Conjugated (BS-4235R-CY5-5)?

KLH conjugated synthetic peptide derived from human ADORA1

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4235R-CY5-5)?

Cy5.5 on BS-4235R-CY5-5: excitation / emission 675nm/694nm.