Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

TREM1 Polyclonal Antibody

The THP-1 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-REM1 antibody (bs-42384R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The THP-1 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-REM1 antibody (bs-42384R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed THP-1 (H) cell; Antibody incubation with (REM1) polyclonal Antibody, unconjugated (bs-42384R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed THP-1 (H) cell; Antibody incubation with (REM1) polyclonal Antibody, unconjugated (bs-42384R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Bioss unconjugated antibody vial

Applications

  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-42384r
Product Name TREM1 Polyclonal Antibody
Applications
FLOW-CYT ICC/IF
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human TREM1 protein
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot Q9NP99
Subcellular location Secreted, Cell membrane
Synonyms Triggering receptor expressed on myeloid cells 1; TREM 1; TREM-1; Triggering receptor expressed on monocytes 1; Triggering receptor TREM 1; Triggering receptor TREM1; Triggering-receptor TREM 1; Triggering-receptor TREM-1; Triggering-receptor TREM1; TREM1_HUMAN; CD354.
Application Dilution
Flow-Cyt =1:50-100
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is TREM1 Polyclonal Antibody (bs-42384R) validated for, and at what dilution?

bs-42384R is validated for Flow-Cyt (Flow-Cyt=1:50-100); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TREM1 Polyclonal Antibody (bs-42384R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bs-42384R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TREM1 Polyclonal Antibody (bs-42384R)?

Recombinant human TREM1 protein

Search our catalog of hundreds of thousands of products