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P53  Polyclonal Antibody

25 ug total protein per lane of various lysates (see on figure) probed with P53 polyclonal antibody, unconjugated (bs-42386R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with P53 polyclonal antibody, unconjugated (bs-42386R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (P53) polyclonal Antibody, unconjugated (bs-42386R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (P53) polyclonal Antibody, unconjugated (bs-42386R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (P53) polyclonal Antibody, unconjugated (bs-42386R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (P53) polyclonal Antibody, unconjugated (bs-42386R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bs-42386r
Product Name P53  Polyclonal Antibody
Applications
WB
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human P53 protei
Clonality Polyclonal
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7157
Swiss Prot P04637
Subcellular location Cytoplasm, Nucleus
Synonyms P53_HUMAN; Cellular tumor antigen p53; TP53; Antigen NY-CO-13;Phosphoprotein p53; Tumor suppressor p53; tumor protein p53; BCC7; LFS1; BMFS5; TRP53; P 53; P-53; wt-p53; Widespread p53; Wtp53; Wt p53;
Application Dilution
WB =1:500-2000

Frequently asked questions

Which applications is P53  Polyclonal Antibody (bs-42386R) validated for, and at what dilution?

bs-42386R is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does P53  Polyclonal Antibody (bs-42386R) react with?

Tested reactivity: Human.

How should bs-42386R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise P53  Polyclonal Antibody (bs-42386R)?

Recombinant human P53 protei

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