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BNIP3 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-4239R-Cy5
Product Name BNIP3 Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BNIP3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 664
Subcellular location Cytoplasm, Cell membrane
Synonyms BCL2 Adenovirus E1B 19kDa Interacting Protein 3; BCL2/adenovirus E1B 19 kDa protein interacting protein 3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP 3; BNIP3; BNIP3_HUMAN; NIP 3; NIP3.
Background Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is BNIP3 Polyclonal Antibody, Cy5 Conjugated (bs-4239R-Cy5) validated for, and at what dilution?

bs-4239R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BNIP3 Polyclonal Antibody, Cy5 Conjugated (bs-4239R-Cy5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken.

How should bs-4239R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BNIP3 Polyclonal Antibody, Cy5 Conjugated (bs-4239R-Cy5)?

KLH conjugated synthetic peptide derived from human BNIP3

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-4239R-Cy5)?

Cy5 on bs-4239R-Cy5: excitation / emission 625,650nm/670nm.