DDIT4 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4240R-Cy3 |
| Product Name | DDIT4 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DDIT4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54541 |
| Subcellular location | Cytoplasm |
| Synonyms | DDIT4; DDIT4_HUMAN; Dig2; DKFZp564O2071; DNA damage inducible transcript 4; DNA damage inducible transcript 4 protein; DNA damage-inducible transcript 4 protein; FLJ20500; H 1 responsive protein RTP801; H 1 responsive RTP801; H-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD 1; REDD-1; REDD1; RP11 442H21.1; RTP801. |
| Background | Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DDIT4 Polyclonal Antibody, Cy3 Conjugated (bs-4240R-Cy3) validated for, and at what dilution?
bs-4240R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DDIT4 Polyclonal Antibody, Cy3 Conjugated (bs-4240R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Horse, Rabbit.
How should bs-4240R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DDIT4 Polyclonal Antibody, Cy3 Conjugated (bs-4240R-Cy3)?
KLH conjugated synthetic peptide derived from human DDIT4
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4240R-Cy3)?
Cy3 on bs-4240R-Cy3: excitation / emission 512,550nm/570,615nm.