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CYP7A1 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
Overview
Catalog # bs-42415r-cy3-100uL
Product Name CYP7A1 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF
Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source Recombinant human CYP7A1 protein
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1581
Swiss Prot P22680
Subcellular location Cell membrane
Synonyms Cholesterol 7 alpha hydroxylase; Cholesterol 7 alpha monooxygenase; cholesterol 7 alpha-monooxygenase; Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase; CP7A; CYP 7; CYP7; CYPVII; Cytochrome P450 7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase); CP7A1_HUMAN
Application Dilution
WB =1:500-2000
IF IF=1:50-200

Frequently asked questions

Which applications is CYP7A1 Polyclonal Antibody, Cy3 Conjugated (bs-42415R-Cy3) validated for, and at what dilution?

bs-42415R-Cy3 is validated for WB (WB=1:500-2000); IF (IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CYP7A1 Polyclonal Antibody, Cy3 Conjugated (bs-42415R-Cy3) react with?

Tested reactivity: Human.

How should bs-42415R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CYP7A1 Polyclonal Antibody, Cy3 Conjugated (bs-42415R-Cy3)?

Recombinant human CYP7A1 protein

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-42415R-Cy3)?

Cy3 on bs-42415R-Cy3: excitation / emission 512,550nm/570,615nm.