NFAT1 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4246R-APC-Cy5.5-100uL |
| Product Name | NFAT1 Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NFAT1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4773 |
| Swiss Prot | Q13469 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | cytoplasmic 2; NF ATp; NF-ATc2; NF-ATp; NFAC2_HUMAN; NFAT 1; NFAT pre existing subunit; NFAT pre-existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1-D; NFATc2; NFATp; Nuclear factor of activated T cells cytoplasmic 2; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2; Nuclear factor of activated T cells pre-existing component; Nuclear factor of activated T-cells; T cell transcription factor NFAT 1; T-cell transcription factor NFAT1; NFAC2_HUMAN. |
| Background | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NFAT1 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-4246R-APC-CY5-5) validated for, and at what dilution?
BS-4246R-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NFAT1 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-4246R-APC-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse.
How should BS-4246R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NFAT1 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-4246R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human NFAT1
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-4246R-APC-CY5-5)?
APC-Cy5.5 on BS-4246R-APC-CY5-5: excitation / emission 650nm/694nm.