PHD1 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4251R-Cy7-100uL |
| Product Name | PHD1 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PHD1/prolyl hydroxylase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 112398 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Egl nine homolog 2; prolyl hydroxylase;EGLN 2; EGLN2; EIT 6; EIT6; H P4H 1; H PH1; HPH1; HPH 3; HPH3; P4H1; PHD 1; PHD1; EGL nine C.elegans homolog 2; EGLN2_HUMAN. |
| Background | PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PHD1 Polyclonal Antibody, Cy7 Conjugated (bs-4251R-Cy7) validated for, and at what dilution?
bs-4251R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PHD1 Polyclonal Antibody, Cy7 Conjugated (bs-4251R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep.
How should bs-4251R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PHD1 Polyclonal Antibody, Cy7 Conjugated (bs-4251R-Cy7)?
KLH conjugated synthetic peptide derived from human PHD1/prolyl hydroxylase
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-4251R-Cy7)?
Cy7 on bs-4251R-Cy7: excitation / emission 743nm/767nm.