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Vesicle docking protein p115 Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-4258R-FITC
Product Name Vesicle docking protein p115 Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Vesicle docking protein p115
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8615
Swiss Prot O60763
Subcellular location Cytoplasm, Cell membrane
Synonyms TAP; VDP; P115; General vesicular transport factor p115; Protein USO1 homolog; Transcytosis-associated protein; Vesicle-docking protein; USO1
Background General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Vesicle docking protein p115 Antibody, FITC Conjugated (bs-4258R-FITC) validated for, and at what dilution?

bs-4258R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Vesicle docking protein p115 Antibody, FITC Conjugated (bs-4258R-FITC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Pig, Horse, Chicken.

How should bs-4258R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Vesicle docking protein p115 Antibody, FITC Conjugated (bs-4258R-FITC)?

KLH conjugated synthetic peptide derived from human Vesicle docking protein p115

What are the excitation and emission wavelengths of the FITC conjugate (bs-4258R-FITC)?

FITC on bs-4258R-FITC: excitation / emission 494nm/518nm.