epithelial Sodium Channel gamma Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4263R-Cy7 |
| Product Name | epithelial Sodium Channel gamma Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ENaCg |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6340 |
| Swiss Prot | P51170 |
| Subcellular location | Cell membrane |
| Synonyms | PHA1; BESC3; ENaCg; SCNEG; ENaCgamma; Amiloride-sensitive sodium channel subunit gamma; Epithelial Na(+) channel subunit gamma; Gamma-ENaC; Gamma-NaCH; Nonvoltage-gated sodium channel 1 subunit gamma; SCNN1G |
| Background | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is epithelial Sodium Channel gamma Polyclonal Antibody, Cy7 Conjugated (bs-4263R-Cy7) validated for, and at what dilution?
bs-4263R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does epithelial Sodium Channel gamma Polyclonal Antibody, Cy7 Conjugated (bs-4263R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Rabbit.
How should bs-4263R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise epithelial Sodium Channel gamma Polyclonal Antibody, Cy7 Conjugated (bs-4263R-Cy7)?
KLH conjugated synthetic peptide derived from human ENaCg
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-4263R-Cy7)?
Cy7 on bs-4263R-Cy7: excitation / emission 743nm/767nm.