CDKN2B/p15 INK4b Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4269R-FITC |
| Product Name | CDKN2B/p15 INK4b Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat, Bovine, Dog, Chicken |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDKN2B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1030 |
| Swiss Prot | P42772 |
| Synonyms | CDK inhibitory protein; CDK4B Inhibitor; CDKN2B; Cyclin dependent kinase 4 inhibitor B; Cyclin Dependent Kinase Inhibitor 2B; Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4; Cyclin-dependent kinases 4 and 6 binding protein; INK4B; MTS 2; MTS2; Multiple Tumor Supressor 2; p14 CDK inhibitor; p14 INK4b; P15; p15 CDK inhibitor; p15 inhibits CDK4; p15 INK4b; P15INK4B; p15INK4B; TP 15; TP15. |
| Background | p15 INK4b / MTS 2 (Mitotic Inhibitor / Suppressor Protein) is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during the cell cycle. Reportedly, p15 INK4b expression is induced approximately 30 fold in human keratinocytes by treatment with TGF beta, suggesting that p15 INK4b may act as an effector of TGF beta mediated cell cycle arrest. p15 INK4b is mapped to chromosome 9p21, a region of frequent loss in a wide variety of cancers. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is CDKN2B/p15 INK4b Polyclonal Antibody, FITC Conjugated (bs-4269R-FITC) validated for, and at what dilution?
bs-4269R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDKN2B/p15 INK4b Polyclonal Antibody, FITC Conjugated (bs-4269R-FITC) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken.
How should bs-4269R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDKN2B/p15 INK4b Polyclonal Antibody, FITC Conjugated (bs-4269R-FITC)?
KLH conjugated synthetic peptide derived from human CDKN2B
What are the excitation and emission wavelengths of the FITC conjugate (bs-4269R-FITC)?
FITC on bs-4269R-FITC: excitation / emission 494nm/518nm.