DBC2 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4274R |
| Product Name | DBC2 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DBC2 |
| Immunogen Range | 631-727/727 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 23221 |
| Swiss Prot | Q9BYZ6 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | DBC2; Rho-related BTB domain-containing protein 2; Deleted in breast cancer 2 gene protein; p83; RHOBTB2; KIAA0717 |
| Background | DBC2 is a member of the recently identified RhoBTB family, which is part of the Rho GTPase family. DBC2 has been implicated as a tumor suppressor in breast and lung cancer. Studies have shown that DBC2 binds to the ubiquitin ligase scaffold Cul3, and that Cul3 regulates DBC2 protein levels by ubiquitinating DBC2 directly, leading to its degradation by the proteasome. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DBC2 Polyclonal Antibody (bs-4274R) validated for, and at what dilution?
bs-4274R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DBC2 Polyclonal Antibody (bs-4274R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Horse.
How should bs-4274R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DBC2 Polyclonal Antibody (bs-4274R)?
KLH conjugated synthetic peptide derived from human DBC2 Immunogen range: 631-727/727.