ELAC2/HPC2 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4276R-RBITC-100uL |
| Product Name | ELAC2/HPC2 Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ELAC2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 60528 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | elaC homolog 2; ElaC homolog protein 2; ELC2; Heredity prostate cancer protein 2; HPC2; putative prostate cancer susceptibility protein HPC2/ELAC2; Ribonuclease Z2; RNase Z2; tRNA 3 endonuclease 2; tRNase Z long form; tRNase Z2; Zinc phosphodiesterase ELAC protein 2. |
| Background | ELAC2 is a zinc phosphodiesterase which displays some tRNA 3' processing endonuclease activity. It is thought to be involved in tRNA maturation, acting by removing a 3' trailer from precursor tRNA. ELAC2 also interacts with activated Smad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expression can suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased risk of prostate cancer. A number of allelic variants have been investigated in hereditary prostate cancer. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ELAC2/HPC2 Polyclonal Antibody, RBITC Conjugated (bs-4276R-RBITC) validated for, and at what dilution?
bs-4276R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ELAC2/HPC2 Polyclonal Antibody, RBITC Conjugated (bs-4276R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Rabbit.
How should bs-4276R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ELAC2/HPC2 Polyclonal Antibody, RBITC Conjugated (bs-4276R-RBITC)?
KLH conjugated synthetic peptide derived from human ELAC2
What are the excitation and emission wavelengths of the RBITC conjugate (bs-4276R-RBITC)?
RBITC on bs-4276R-RBITC: excitation / emission 540nm/625nm.