FAT Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4278R-BF680-100uL |
| Product Name | FAT Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FAT/CDHF7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2195 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Cadherin family member 7; cadherin family member 7 precursor; cadherin ME5; Cadherin related tumor suppressor; Cadherin related tumor suppressor homolog; cadherin related tumor suppressor homolog precursor; Cadherin related tumor suppressor homolog precursor FAT protein homolog; CDHF 7; CDHF7; FAT 1; FAT tumor suppressor 1; FAT tumor suppressor homolog 1; FAT1; hFat 1; hFAT; hFat1; homolog of Drosophila FAT protein; homolog of Drosophila FAT protein precursor; homolog of Drosophila tumor suppressor FAT precursor; ME 5; ME5; Protein fat homolog. |
| Background | This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq]. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FAT Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4278R-BF680) validated for, and at what dilution?
bs-4278R-BF680 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FAT Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4278R-BF680) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse.
How should bs-4278R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FAT Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4278R-BF680)?
KLH conjugated synthetic peptide derived from human FAT/CDHF7
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-4278R-BF680)?
AbBy Fluor-680 on bs-4278R-BF680: excitation / emission 679nm/702nm.