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Ran Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Pig
  • Chicken
Overview
Catalog # bs-4282R-Cy5.5-100uL
Product Name Ran Polyclonal Antibody, Cy5.5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Pig, Chicken
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Ran
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5901
Swiss Prot P62826
Subcellular location Cytoplasm, Nucleus
Synonyms Androgen receptor associated protein 24; Androgen receptor-associated protein 24; ARA 24; ARA24; Gsp1; GTP binding nuclear protein RAN; GTP-binding nuclear protein Ran; GTPase Ran; LPS; ran; RAN member RAS oncogene family; RAN_HUMAN; Ras like protein TC4; Ras related nuclear protein; Ras-like protein TC4; Ras-related nuclear protein; RASL2 8; TC 4; TC4.
Background GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Ran Polyclonal Antibody, Cy5.5 Conjugated (BS-4282R-CY5-5) validated for, and at what dilution?

BS-4282R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Ran Polyclonal Antibody, Cy5.5 Conjugated (BS-4282R-CY5-5) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Chicken.

How should BS-4282R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Ran Polyclonal Antibody, Cy5.5 Conjugated (BS-4282R-CY5-5)?

KLH conjugated synthetic peptide derived from human Ran

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4282R-CY5-5)?

Cy5.5 on BS-4282R-CY5-5: excitation / emission 675nm/694nm.

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