5HT3B receptor Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4289R-APC-Cy5.5-100uL |
| Product Name | 5HT3B receptor Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Rabbit |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human 5HTR3B/5HT3B receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9177 |
| Swiss Prot | O95264 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 5-HT3B; 5-hydroxytryptamine receptor 3B; 5-HT3-B; Serotonin receptor 3B; HTR3B |
| Background | This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is 5HT3B receptor Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-4289R-APC-CY5-5) validated for, and at what dilution?
BS-4289R-APC-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 5HT3B receptor Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-4289R-APC-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Rabbit.
How should BS-4289R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 5HT3B receptor Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-4289R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human 5HTR3B/5HT3B receptor
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-4289R-APC-CY5-5)?
APC-Cy5.5 on BS-4289R-APC-CY5-5: excitation / emission 650nm/694nm.