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MACC1 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
Overview
Catalog # bs-4293R-FITC
Product Name MACC1 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MACC1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 346389
Swiss Prot Q6ZN28
Subcellular location Cytoplasm, Nucleus
Synonyms 7A5; SH3BP4L; Metastasis-associated in colon cancer protein 1; SH3 domain-containing protein 7a5; MACC1
Background Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MACC1 Polyclonal Antibody, FITC Conjugated (bs-4293R-FITC) validated for, and at what dilution?

bs-4293R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MACC1 Polyclonal Antibody, FITC Conjugated (bs-4293R-FITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow.

How should bs-4293R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MACC1 Polyclonal Antibody, FITC Conjugated (bs-4293R-FITC)?

KLH conjugated synthetic peptide derived from human MACC1

What are the excitation and emission wavelengths of the FITC conjugate (bs-4293R-FITC)?

FITC on bs-4293R-FITC: excitation / emission 494nm/518nm.