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CDC40 Polyclonal Antibody, Cy3 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-4295R-Cy3
Product Name CDC40 Polyclonal Antibody, Cy3 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CDC40
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 51362
Swiss Prot O60508
Subcellular location Nucleus
Synonyms EHB3; PRP17; PRPF17; Pre-mRNA-processing factor 17; Cell division cycle 40 homolog; EH-binding protein 3; PRP17 homolog; hPRP17; CDC40
Background Associates with the spliceosome late in the splicing pathway and may function in the second step of pre-mRNA splicing.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CDC40 Polyclonal Antibody, Cy3 Conjugated (bs-4295R-Cy3) validated for, and at what dilution?

bs-4295R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CDC40 Polyclonal Antibody, Cy3 Conjugated (bs-4295R-Cy3) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken.

How should bs-4295R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CDC40 Polyclonal Antibody, Cy3 Conjugated (bs-4295R-Cy3)?

KLH conjugated synthetic peptide derived from human CDC40

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4295R-Cy3)?

Cy3 on bs-4295R-Cy3: excitation / emission 512,550nm/570,615nm.