MHC Class II Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4298R-APC-Cy7-100uL |
| Product Name | MHC Class II Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen gamma chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 972 |
| Swiss Prot | P04233 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | II; DHLAG; HLADG; Ia-GAMMA; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; p33; CD74 |
| Background | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MHC Class II Polyclonal Antibody, APC-Cy7 Conjugated (bs-4298R-APC-Cy7) validated for, and at what dilution?
bs-4298R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MHC Class II Polyclonal Antibody, APC-Cy7 Conjugated (bs-4298R-APC-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-4298R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MHC Class II Polyclonal Antibody, APC-Cy7 Conjugated (bs-4298R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen gamma chain
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-4298R-APC-Cy7)?
APC-Cy7 on bs-4298R-APC-Cy7: excitation / emission 650nm/780nm.