PRL Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PRL) Polyclonal Antibody, Unconjugated (bs-43099R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PRL) Polyclonal Antibody, Unconjugated (bs-43099R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Lane 1: Recombinant human PRL protein, N-His (HEK293) (bs-43099P) probed with PRL Polyclonal Antibody, Unconjugated (bs-43099R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant human PRL protein, N-His (HEK293) (bs-43099P) probed with PRL Polyclonal Antibody, Unconjugated (bs-43099R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Predicted Reactivity

Human

Overview
Catalog #	bs-43099r
Product Name	PRL Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human PRL protein
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5617
Swiss Prot	P01236
Synonyms	Luteotropic Hormone; Mammotropin; OTTHUMP00000017727; PRL; Prolactin; Prolactin precursor; PRL_HUMAN
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500