MAP1LC3A Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4309R-BF647-100uL |
| Product Name | MAP1LC3A Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MAP LC3 Alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54901, 84557 |
| Swiss Prot | Q9H492, Q9GZQ8 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Microtubule-associated proteins 1A/B light chain 3A; Microtubule-associated proteins 1A/B light chain 3B; Microtubule-associated proteins 1Beta light chain 3A; Microtubule-associated proteins 1Beta light chain 3A; MAP1B LC3 A; MAP LC3 Beta; MAP-LC3 Beta; MAP1 light chain 3-like protein 1.MAP1LC3B.MLP3A_HUMAN |
| Background | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3 Beta) and MAP-light chain 3 alpha (MAP-LC3 alpha) are subunits of both MAP1A and MAP1B. MAP-LC3M Beta, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3 Beta, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3? untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3 Beta may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle. |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is MAP1LC3A Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-4309R-BF647) validated for, and at what dilution?
bs-4309R-BF647 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAP1LC3A Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-4309R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken.
How should bs-4309R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAP1LC3A Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-4309R-BF647)?
KLH conjugated synthetic peptide derived from human MAP LC3 Alpha
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-4309R-BF647)?
AbBy Fluor-647 on bs-4309R-BF647: excitation / emission 650nm/665nm.