phospho-PAK1 (Ser204) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4318R-BF750-100uL |
| Product Name | phospho-PAK1 (Ser204) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human PAK1 around the phosphorylation site of Ser204 |
| Modification Site | Ser204 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5058 |
| Swiss Prot | Q13153 |
| Subcellular location | Cytoplasm |
| Synonyms | PAK1 (phospho S204); p-PAK1 (phospho S204); ADRB2; Alpha PAK; Alpha-PAK; MGC130000; MGC130001; p21 activated kinase 1; p21 protein (Cdc42/Rac) activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p65 PAK; p65-PAK; P68-PAK; PAK alpha; PAK-1; Pak1; PAK1_HUMAN; Paka; PAKalpha; Protein kinase MUK2; Rac/p21-activated kinase; Serine/threonine-protein kinase PAK 1; STE20 homolog yeast. |
| Background | The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PAK1 (Ser204) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-4318R-BF750) validated for, and at what dilution?
bs-4318R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PAK1 (Ser204) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-4318R-BF750) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-4318R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PAK1 (Ser204) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-4318R-BF750)?
KLH conjugated synthesised phosphopeptide derived from human PAK1 around the phosphorylation site of Ser204
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-4318R-BF750)?
AbBy Fluor-750 on bs-4318R-BF750: excitation / emission 749nm/775nm.