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CD28 Polyclonal Antibody

Lane 1: Recombinant human CD28 protein, C-hFc (HEK193)(bs-43587P) probed with CD28 Polyclonal Antibody, Unconjugated (bs-43587R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Lane 1: Recombinant human CD28 protein, C-hFc (HEK193)(bs-43587P) probed with CD28 Polyclonal Antibody, Unconjugated (bs-43587R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD28) Polyclonal Antibody, Unconjugated (bs-43587R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD28) Polyclonal Antibody, Unconjugated (bs-43587R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human

Predicted Reactivity

  • Dog
  • Horse
  • Rabbit
Overview
Catalog # bs-43587r
Product Name CD28 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human
Predicted Reactivity Dog, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source 19-152/220
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 940
Swiss Prot P31041
Subcellular location Cell membrane
Synonyms CD 28; CD28 antigen; CD28 molecule; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; Tp44; CD28_HUMAN; CD28_MOUSE.
Application Dilution
WB
IHC-P
IHC-F
IF

Frequently asked questions

Which applications is CD28 Polyclonal Antibody (bs-43587R) validated for, and at what dilution?

bs-43587R is validated for WB, IHC-P, IHC-F, IF.

Which species does CD28 Polyclonal Antibody (bs-43587R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Horse, Rabbit.

How should bs-43587R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD28 Polyclonal Antibody (bs-43587R)?

19-152/220

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