EphA3 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-43620R-APC-100uL |
| Product Name | EphA3 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
|
| Reactivity | Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | Recombinant human EphA3 protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2044 |
| Swiss Prot | P54764 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | EK4; End3; Eph receptor A3; EPH-like kinase 4; EPH-like tyrosine kinase 1; EphA3; Ephrin receptor EphA3; Ephrin type-A receptor 3; ETK 1; ETK; ETK1; HEK 4; HEK; Hek4; Human embryo kinase; Mek4; MGC109882; Receptor tyrosine kinase HEK; Tyro 4; Tyro4; Tyrosine protein kinase receptor ETK 1; Tyrosine-protein kinase TYRO4; AW492086; Cek4; EC 2.7.10.1; EPHA3_HUMAN. |
| Background | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development. |
| Application Dilution | |
| WB | =1:500-2000 |
Frequently asked questions
Which applications is EphA3 Polyclonal Antibody, APC Conjugated (bs-43620R-APC) validated for, and at what dilution?
bs-43620R-APC is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EphA3 Polyclonal Antibody, APC Conjugated (bs-43620R-APC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-43620R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EphA3 Polyclonal Antibody, APC Conjugated (bs-43620R-APC)?
Recombinant human EphA3 protein
What are the excitation and emission wavelengths of the APC conjugate (bs-43620R-APC)?
APC on bs-43620R-APC: excitation / emission 650nm/660nm.