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ZNF710 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ZNF710) Polyclonal Antibody, Unconjugated (bs-4373R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ZNF710) Polyclonal Antibody, Unconjugated (bs-4373R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-4373r
Product Name ZNF710 Polyclonal Antibody
Applications
IHC-P IHC-F IF ICC/IF
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ZNF710
Immunogen Range 401-500/664
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 374655
Swiss Prot Q8N1W2
Subcellular location Nucleus
Synonyms DKFZp547K1113; FLJ00306; FLJ37393; MGC163413; Zinc finger protein 710; ZN710_HUMAN; Znf710.
Background ZNF710 (Zinc Finger Protein 710) is a Protein Coding gene. Among its related pathways are Gene Expression. GO annotations related to this gene include nucleic acid binding and transcription cofactor activity. An important paralog of this gene is ZNF366.
Application Dilution
IHC-P =1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500
ICC/IF ICC/IF=1:50

Frequently asked questions

Which applications is ZNF710 Polyclonal Antibody (bs-4373R) validated for, and at what dilution?

bs-4373R is validated for IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500); ICC/IF (ICC/IF=1:50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ZNF710 Polyclonal Antibody (bs-4373R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse.

How should bs-4373R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZNF710 Polyclonal Antibody (bs-4373R)?

KLH conjugated synthetic peptide derived from human ZNF710 Immunogen range: 401-500/664.

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