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CXCL13/BCA1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-CXCL13 Polyclonal Antibody, Unconjugated (bs-4509R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-CXCL13 Polyclonal Antibody, Unconjugated (bs-4509R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human Jurkat cell lysates probed with CXCL13 Polyclonal Antibody, Unconjugated (bs-2553R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n
Lane 1: Human Jurkat cell lysates probed with CXCL13 Polyclonal Antibody, Unconjugated (bs-2553R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n
Blank control\uff08black line\uff09:HepG2. <BR> \r\nPrimary Antibody (green line): Rabbit Anti-CXCL13 antibody (bs-4509R) <BR>\r\nDilution:1ug\/Test; <BR> \r\nSecondary Antibody\uff08white blue line\uff09: Goat anti-rabbit IgG-AF488<BR>\r\nDilution: 0.5ug\/Test. <br>\r\nIsotype control\uff08orange line\uff09: Normal Rabbit IgG<br>\r\nProtocol<BR>\r\nThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control\uff08black line\uff09:HepG2. <BR> \r\nPrimary Antibody (green line): Rabbit Anti-CXCL13 antibody (bs-4509R) <BR>\r\nDilution:1ug\/Test; <BR> \r\nSecondary Antibody\uff08white blue line\uff09: Goat anti-rabbit IgG-AF488<BR>\r\nDilution: 0.5ug\/Test. <br>\r\nIsotype control\uff08orange line\uff09: Normal Rabbit IgG<br>\r\nProtocol<BR>\r\nThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Dog
Overview
Catalog # bs-4509R
Product Name CXCL13/BCA1 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Dog
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CXCL13/BCA1
Immunogen Range 23-109/109
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 10563
Swiss Prot O43927
Subcellular location Secreted
Synonyms BLC; Angie; BCA-1; BLR1L; ANGIE2; Scyb13; 4631412M8Rik; C-X-C motif chemokine 13; B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; Cxcl13
Background Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in directing the migration of B-lymphocytes to follicles in secondary lymphoid organs.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CXCL13/BCA1 Polyclonal Antibody (bs-4509R) validated for, and at what dilution?

bs-4509R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CXCL13/BCA1 Polyclonal Antibody (bs-4509R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Dog.

How should bs-4509R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CXCL13/BCA1 Polyclonal Antibody (bs-4509R)?

KLH conjugated synthetic peptide derived from human CXCL13/BCA1 Immunogen range: 23-109/109.

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