TUBB3 (Neuronal Marker) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4512R-Cy5.5-100uL |
| Product Name | TUBB3 (Neuronal Marker) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Neuron specific beta III Tubulin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10381 |
| Swiss Prot | Q13509 |
| Subcellular location | Cytoplasm |
| Synonyms | CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3 |
| Background | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TUBB3 (Neuronal Marker) Polyclonal Antibody, Cy5.5 Conjugated (BS-4512R-CY5-5) validated for, and at what dilution?
BS-4512R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TUBB3 (Neuronal Marker) Polyclonal Antibody, Cy5.5 Conjugated (BS-4512R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Rabbit.
How should BS-4512R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TUBB3 (Neuronal Marker) Polyclonal Antibody, Cy5.5 Conjugated (BS-4512R-CY5-5)?
KLH conjugated synthetic peptide derived from human Neuron specific beta III Tubulin
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4512R-CY5-5)?
Cy5.5 on BS-4512R-CY5-5: excitation / emission 675nm/694nm.