TGF Beta 1+2+3 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4538R-100uL |
| Product Name | TGF Beta 1+2+3 Polyclonal Antibody |
| Applications |
WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will preferentially react with TGFB1, but will also detect TGFB2 (93%) and TGFB3 (86%) based upon sequence similarity. |
| Reactivity | Human, Mouse, Rat, Rabbit |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, GuineaPig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TGF Beta 1 |
| Immunogen Range | 301-350/390 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7040 |
| Swiss Prot | P01137 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | CED; LAP; DPD1; TGFB; TGFbeta; Transforming growth factor beta-1; TGF-beta-1; TGFB1; TGFB2; TGFB3 |
| Background | Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TGF Beta 1+2+3 Polyclonal Antibody (bs-4538R) validated for, and at what dilution?
bs-4538R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TGF Beta 1+2+3 Polyclonal Antibody (bs-4538R) react with?
Tested reactivity: Human, Mouse, Rat, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, GuineaPig.
How should bs-4538R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TGF Beta 1+2+3 Polyclonal Antibody (bs-4538R)?
KLH conjugated synthetic peptide derived from human TGF Beta 1 Immunogen range: 301-350/390.