Pectinase Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4550R-FITC |
| Product Name | Pectinase Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Others |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | Pectinase from Aspergillus niger |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Pectinesterase/pectinesterase inhibitor 18; AtPMEpcrA; Contains: Pectinesterase inhibitor 18; Pectin methylesterase inhibitor 18; Contains: Bifunctional pectinesterase 18/rRNA N-glycosylase; PE 18; Pectin methylesterase 18; Pectin methylesterase 4; AtPME4. |
| Background | Pectinase is a multi-enzyme complex that decomposes pectin, usually including protopectinase, pectin methyl ester hydrolase, and pectin acidase. Through their combined action, pectin can be completely decomposed. Natural pectin is converted into water-soluble pectin under the action of protopectinase; pectin is catalyzed by pectin methyl ester hydrolase to remove the methyl ester group to produce pectin acid; pectin acid is degraded by pectin hydrolases and pectin lyases to produce galacturonic acid. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Pectinase Polyclonal Antibody, FITC Conjugated (bs-4550R-FITC) validated for, and at what dilution?
bs-4550R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-4550R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Pectinase Polyclonal Antibody, FITC Conjugated (bs-4550R-FITC)?
Pectinase from Aspergillus niger
What are the excitation and emission wavelengths of the FITC conjugate (bs-4550R-FITC)?
FITC on bs-4550R-FITC: excitation / emission 494nm/518nm.