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Bcl-2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rabbit pancreas labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit pancreas labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Mouse splenocytes probed with Rabbit Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-4563R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-4563R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Bcl-2 antibody (bs-4563R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Bcl-2 antibody (bs-4563R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-4563R-100uL
Product Name Bcl-2 Polyclonal Antibody
Applications
WB FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Bcl-2
Immunogen Range 161-239/239
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 596
Swiss Prot P10415
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Bcl-2; PPP1R5; Apoptosis regulator Bcl-2; BCL2
Background Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Bcl-2 Polyclonal Antibody (bs-4563R) validated for, and at what dilution?

bs-4563R is validated for WB (1:300-5000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Bcl-2 Polyclonal Antibody (bs-4563R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-4563R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Bcl-2 Polyclonal Antibody (bs-4563R)?

KLH conjugated synthetic peptide derived from human Bcl-2 Immunogen range: 161-239/239.

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