VEGFA Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4572R-FITC |
| Product Name | VEGFA Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat, GuineaPig |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VEGF-A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7422 |
| Swiss Prot | P15692 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | VPF; VEGF; MVCD1; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VEGFA |
| Background | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is VEGFA Polyclonal Antibody, FITC Conjugated (bs-4572R-FITC) validated for, and at what dilution?
bs-4572R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VEGFA Polyclonal Antibody, FITC Conjugated (bs-4572R-FITC) react with?
Tested reactivity: Human, Rat, GuineaPig. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Chicken.
How should bs-4572R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VEGFA Polyclonal Antibody, FITC Conjugated (bs-4572R-FITC)?
KLH conjugated synthetic peptide derived from human VEGF-A
What are the excitation and emission wavelengths of the FITC conjugate (bs-4572R-FITC)?
FITC on bs-4572R-FITC: excitation / emission 494nm/518nm.