IL-6 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4587R-Cy5.5-100uL |
| Product Name | IL-6 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Bovine, Pig, Rabbit |
| Predicted Reactivity | Cow, Sheep, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IL-6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3569 |
| Swiss Prot | P05231 |
| Subcellular location | Secreted |
| Synonyms | HGF; HSF; BSF2; IL-6; IFNB2; Interleukin-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6 |
| Background | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IL-6 Polyclonal Antibody, Cy5.5 Conjugated (BS-4587R-CY5-5) validated for, and at what dilution?
BS-4587R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IL-6 Polyclonal Antibody, Cy5.5 Conjugated (BS-4587R-CY5-5) react with?
Tested reactivity: Human, Mouse, Bovine, Pig, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Horse.
How should BS-4587R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IL-6 Polyclonal Antibody, Cy5.5 Conjugated (BS-4587R-CY5-5)?
KLH conjugated synthetic peptide derived from human IL-6
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4587R-CY5-5)?
Cy5.5 on BS-4587R-CY5-5: excitation / emission 675nm/694nm.