CDKN2A/p16INK4a Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4592R-BF647-100uL |
| Product Name | CDKN2A/p16INK4a Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Targets p16INK4a |
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDKN2A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1029 |
| Swiss Prot | P42771 |
| Subcellular location | Nucleus |
| Synonyms | Cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A; p12; CDKN2; MTS1. |
| Background | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CDKN2A/p16INK4a Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-4592R-BF647) validated for, and at what dilution?
bs-4592R-BF647 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDKN2A/p16INK4a Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-4592R-BF647) react with?
Tested reactivity: Human.
How should bs-4592R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDKN2A/p16INK4a Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-4592R-BF647)?
KLH conjugated synthetic peptide derived from human CDKN2A
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-4592R-BF647)?
AbBy Fluor-647 on bs-4592R-BF647: excitation / emission 650nm/665nm.