Collagen IV Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4595R-APC-Cy7-100uL |
| Product Name | Collagen IV Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1282 |
| Swiss Prot | P02462 |
| Subcellular location | Secreted, Cell membrane, Extracellular matrix |
| Synonyms | ICH; HANAC; POREN1; arresten; Collagen alpha-1(IV) chain; COL4A1 |
| Background | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Collagen IV Polyclonal Antibody, APC-Cy7 Conjugated (bs-4595R-APC-Cy7) validated for, and at what dilution?
bs-4595R-APC-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Collagen IV Polyclonal Antibody, APC-Cy7 Conjugated (bs-4595R-APC-Cy7) react with?
Tested reactivity: Human.
How should bs-4595R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Collagen IV Polyclonal Antibody, APC-Cy7 Conjugated (bs-4595R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-4595R-APC-Cy7)?
APC-Cy7 on bs-4595R-APC-Cy7: excitation / emission 650nm/780nm.