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HOMER2 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-4632R-Cy7
Product Name HOMER2 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Cow, Pig, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HOMER2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9455
Swiss Prot Q9NSB8
Subcellular location Cytoplasm, Cell membrane, Extracellular matrix
Synonyms Cupidin; HOME2_HUMAN; HOMER 2; HOMER 2A; HOMER 2B; Homer homolog 2 Drosophila; Homer homolog 3; Homer neuronal immediate early gene 2; Homer protein homolog 2; Homer-2; Homer2; HOMER2A; HOMER2B; Vesl 2; Vesl2.
Background Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is HOMER2 Polyclonal Antibody, Cy7 Conjugated (bs-4632R-Cy7) validated for, and at what dilution?

bs-4632R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HOMER2 Polyclonal Antibody, Cy7 Conjugated (bs-4632R-Cy7) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig, Rabbit.

How should bs-4632R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HOMER2 Polyclonal Antibody, Cy7 Conjugated (bs-4632R-Cy7)?

KLH conjugated synthetic peptide derived from human HOMER2

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-4632R-Cy7)?

Cy7 on bs-4632R-Cy7: excitation / emission 743nm/767nm.