Mst1(Thr183) Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4635r-bf594 |
| Product Name | Mst1(Thr183) Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat, Bovine |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Mst1 around the phosphorylation site of Thr183. |
| Modification Site | Thr183 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4485 |
| Synonyms | Mst1T183; STK4; Krs2; Mammalian STE20 like protein kinase 1; Mammalian sterile 20 like 1; Serine/threonine protein kinase Krs 2; STE20 like kinase MST1; STK4Thr183/STK3Thr180; Mst1/2Thr183/Thr180; STK4/3Thr183/Thr180. |
| Background | MST1/STK4 encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Mst1(Thr183) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-4635R-BF594) validated for, and at what dilution?
bs-4635R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mst1(Thr183) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-4635R-BF594) react with?
Tested reactivity: Human, Mouse, Rat, Bovine.
How should bs-4635R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Mst1(Thr183) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-4635R-BF594)?
KLH conjugated synthetic phosphopeptide derived from human Mst1 around the phosphorylation site of Thr183.
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-4635R-BF594)?
AbBy Fluor-594 on bs-4635R-BF594: excitation / emission 590nm/617nm.