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CRLF2 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-4652R-RBITC-100uL
Product Name CRLF2 Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Rat
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse CRLF2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 64109
Subcellular location Secreted, Cell membrane
Synonyms CRL 2; CRL2; CRLF 2; Crlf2; CRLF2_MOUSE; CRLF2Y; Cytokine receptor CRL2 precusor; Cytokine receptor like 2; Cytokine receptor like factor 2; Cytokine receptor like factor 2 precursor; Cytokine receptor-like 2; Cytokine receptor-like factor 2; IL XR; IL-XR; ILXR; Thymic stromal derived lymphopoietin receptor; Thymic stromal lymphopoietin protein receptor; Thymic stromal lymphopoietin receptor; TSLP receptor; TSLPR.
Background Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.Tissue specificity: Expressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CRLF2 Polyclonal Antibody, RBITC Conjugated (bs-4652R-RBITC) validated for, and at what dilution?

bs-4652R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CRLF2 Polyclonal Antibody, RBITC Conjugated (bs-4652R-RBITC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-4652R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CRLF2 Polyclonal Antibody, RBITC Conjugated (bs-4652R-RBITC)?

KLH conjugated synthetic peptide derived from mouse CRLF2

What are the excitation and emission wavelengths of the RBITC conjugate (bs-4652R-RBITC)?

RBITC on bs-4652R-RBITC: excitation / emission 540nm/625nm.

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