CRLF2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4652R-Cy3 |
| Product Name | CRLF2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse CRLF2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 64109 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | CRL 2; CRL2; CRLF 2; Crlf2; CRLF2_MOUSE; CRLF2Y; Cytokine receptor CRL2 precusor; Cytokine receptor like 2; Cytokine receptor like factor 2; Cytokine receptor like factor 2 precursor; Cytokine receptor-like 2; Cytokine receptor-like factor 2; IL XR; IL-XR; ILXR; Thymic stromal derived lymphopoietin receptor; Thymic stromal lymphopoietin protein receptor; Thymic stromal lymphopoietin receptor; TSLP receptor; TSLPR. |
| Background | Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.Tissue specificity: Expressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CRLF2 Polyclonal Antibody, Cy3 Conjugated (bs-4652R-Cy3) validated for, and at what dilution?
bs-4652R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CRLF2 Polyclonal Antibody, Cy3 Conjugated (bs-4652R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-4652R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CRLF2 Polyclonal Antibody, Cy3 Conjugated (bs-4652R-Cy3)?
KLH conjugated synthetic peptide derived from mouse CRLF2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4652R-Cy3)?
Cy3 on bs-4652R-Cy3: excitation / emission 512,550nm/570,615nm.