phospho-MARK4 (Ser423) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4660R-APC |
| Product Name | phospho-MARK4 (Ser423) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Horse |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MARK4 around the phosphorylation site of Ser423. |
| Modification Site | Ser423 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57787 |
| Subcellular location | Cytoplasm |
| Synonyms | MARK4phospho 423; MARK 4; MARK-4; MARK 4L; MARK L1; MARK4; MARK4 serine / threonine protein kinase; MARK4 serine/threonine protein kinase; MARK4_HUMAN; MARKL 1; MARKL1; MAP / microtubule affinity regulating kinase 4; MAP / microtubule affinity regulating kinase 4L; MAP/microtubule affinity regulating kinase 4; MAP/microtubule affinity regulating kinase like 1; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase-like 1; Putative protein product of Nbla00650; FLJ90097; KIAA1860; mKIAA1860. |
| Background | This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-MARK4 (Ser423) Polyclonal Antibody, APC Conjugated (bs-4660R-APC) validated for, and at what dilution?
bs-4660R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MARK4 (Ser423) Polyclonal Antibody, APC Conjugated (bs-4660R-APC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse.
How should bs-4660R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MARK4 (Ser423) Polyclonal Antibody, APC Conjugated (bs-4660R-APC)?
KLH conjugated synthetic phosphopeptide derived from human MARK4 around the phosphorylation site of Ser423.
What are the excitation and emission wavelengths of the APC conjugate (bs-4660R-APC)?
APC on bs-4660R-APC: excitation / emission 650nm/660nm.