Histone H4 (Acetyl K16) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4707R-APC-Cy7-100uL |
| Product Name | Histone H4 (Acetyl K16) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Acetyl-Histone H4(K17) |
| Modification Site | K17 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 121504 |
| Swiss Prot | P62805 |
| Subcellular location | Nucleus |
| Synonyms | H4/p; Histone H4; HIST1H4A; H4/A; H4FA |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Histone H4 (Acetyl K16) Polyclonal Antibody, APC-Cy7 Conjugated (bs-4707R-APC-Cy7) validated for, and at what dilution?
bs-4707R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H4 (Acetyl K16) Polyclonal Antibody, APC-Cy7 Conjugated (bs-4707R-APC-Cy7) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Rabbit.
How should bs-4707R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H4 (Acetyl K16) Polyclonal Antibody, APC-Cy7 Conjugated (bs-4707R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human Acetyl-Histone H4(K17)
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-4707R-APC-Cy7)?
APC-Cy7 on bs-4707R-APC-Cy7: excitation / emission 650nm/780nm.